FIG. 1.

MTA treatment reduces histone modifications in HeLa cells. (A) Cells were treated with 1 mM MTA for 24 h and then infected with HSV-1 for 3 h. Histones were extracted from cells, and Western blot analysis was performed with H3K4me3 and H3K9me2 antibodies. (B) After treatment with 1 mM MTA for 24 h, lactate dehydrogenase release was measured as the index for cytotoxicity. DMSO, dimethyl sulfoxide. (C) Cells were infected with HSV-1 for 1, 3, 6, and 10 h. Equal amounts of mRNA from each sample were reverse transcribed, and the level of 28S RNA was analyzed by quantitative real-time PCR.