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siRNA-mediated knockdown of Set1 and Set7/9. Cells were transfected with two rounds of siRNA against luciferase (control) (white bar), Set1 (shaded bar), or Set7/9 (black bar) for 48 h. (A) mRNA levels for Set1 and Set7/9 were measured and normalized to glyceraldehyde-3-phosphate dehydrogenase. (B) Western blot analysis was performed with Set1 or Set7/9 antibody.
