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Effect of peptide-loading complex on the formation of m4/gp34-Kb complexes during Ad-m4 infection. B6 MEFs or TAP−/−, tapasin−/− (Tpn−/−), or β2m−/− cells were infected with Ad-m4 and labeled overnight with 35S. Additional B6 MEFs were infected with MCMV to confirm the presence of comparable expression levels of m4/gp34 between MCMV infection and Ad-m4 infection. Sequential IPs were performed with anti-p8 and anti-m4/gp34.
