FIG. 2.

Roscovitine treatment of HCMV-infected cells reduces the production of extracellular virus particles and has little effect on infectivity. Tissue culture supernatants from infected cells treated with 20 μM Roscovitine or DMSO were used to determine the titer and amount of viral DNA released into the media over time. DNA was isolated from extracellular virus particles as described in Materials and Methods and used as template for real-time PCR to determine the quantity of extracellular virions released from DMSO- or Roscovitine-treated cells. For simplicity, only values for days in which the peak titer was achieved for three independent experiments were used to determine the average (AVG) difference (n-fold) in titer and extracellular DNA between controls and drug-treated samples. Error bars represent the standard deviations between experiments.