TABLE 1.

Mutations on H9 and the CCHH zinc finger motif

^aRecombinant viruses were rescued by eight-plasmid transfection of 293T cells. MDCK cells were then infected with transfectant virus at an MOI of 0.001 and maintained in VGM containing 0.5 μg of trypsin/ml as described in Materials and Methods. Supernatants were collected at 48 h p.i. and assayed for PFU/milliliter. Data represent mean values (n = 3). Asterisk, P < 0.05 (versus WT).

^bPlaque sizes for different mutant viruses on MDCK cells. Plaques were visualized at day 3, and diameters of 10 plaques were measured (millimiters). Data represent mean values (n = 10). Asterisk, P < 0.05 (versus WT).

^cResults from these mutants in set I agree with our previous result (25).

^dA new triple mutant in the CCHH motif.

^eRecombinant virus could not be rescued.