TABLE 2.
Survival data for BALB/c mice after WT and mutant virus infection
| Virus | Expt | No. of survivors/total no. (MDD)a for infectious dose (PFU/mouse) of:
|
Virulence relative to WTb | |||
|---|---|---|---|---|---|---|
| 106 | 105 | 104 | 103 | |||
| WT | ||||||
| CCHH | 1 | 0/5 (6.6 ± 1.1) | 0/5 (8.0 ± 1.7) | 0/5 (9.2 ± 1.1) | 5/5 (>21) | |
| 2 | 0/5 (6.2 ± 1.4) | 0/5 (7.6 ± 1.5) | 0/5 (9.0 ± 1.2) | 3/5 (10.0 ± 0.0) | ||
| 3 | 0/6 (6.7 ± 1.8) | 0/5 (7.4 ± 0.5) | 0/5 (8.6 ± 0.5) | 1/5 (12.8 ± 4.2) | ||
| Single mutations | ||||||
| CCAH | 1 | 0/5 (5.4 ± 0.5) | 0/5 (6.8 ± 0.4) | 0/5 (8.0 ± 0.7) | 1/5 (9.0 ± 1.2) | Same |
| 2 | 0/5 (5.5 ± 0.8) | 1/5 (7.5 ± 0.5) | 0/5 (9.2 ± 1.1) | 5/5 (>21) | ||
| CAHH | 1 | 0/5 (8.4 ± 0.9) | 1/5 (11.5 ± 6.4) | 1/5 (12.8 ± 1.7) | 5/5 (>21) | Same |
| Double mutations | ||||||
| CCAA | 1 | 0/5 (6.7 ± 1.0) | 0/5 (8.6 ± 1.5) | 4/5 (12.0) | 5/5 (>21) | 10× less |
| CAHA | 1 | 0/5 (6.2 ± 0.8) | 0/5 (7.0 ± 0.7) | 1/5 (9.0 ± 0.0) | 4/5 (10.0) | Same |
| CAAH | 1 | 0/5 (7.0 ± 1.4) | 0/5 (8.4 ± 1.1) | 4/5 (13.0) | 5/5 (>21) | 10× less |
| ACHA | 1 | 0/5 (7.0 ± 1.0) | 0/5 (8.0 ± 0.0) | 1/5 (9.5 ± 1.7) | 5/5 (>21) | Same |
| ACAH | 1 | 5/5 (>21) | 5/5 (>21) | 5/5 (>21) | 5/5 (>21) | >100× less |
| 2 | 6/6 (>21) | 5/5 (>21) | 5/5 (>21) | 4/5 (12.0) | ||
| 3 | 2/5 (9.3 ± 0.6) | 3/5 (10.5 ± 3.5) | 5/5 (>21) | 5/5 (>21) | ||
| AAHH | 1 | 0/5 (8.2 ± 1.4) | 0/5 (9.2 ± 1.8) | 4/5 (9.0) | 5/5 (>21) | 10× less |
| Triple mutations | ||||||
| CAAA | 1 | 0/5 (7.8 ± 0.4) | 0/5 (10.6 ± 1.1) | 4/5 (11.0) | 4/5 (11.0) | |
| 2 | 0/5 (7.8 ± 1.5) | 0/5 (13.4 ± 3.2) | 5/5 (>21) | 5/5 (>21) | 10× less | |
| ACAA | 1 | 0/5 (7.6 ± 0.5) | 3/5 (12.5 ± 0.7) | 5/5 (>21) | 5/5 (>21) | 100× less |
| 2 | 2/5 (13.0 ± 2.0) | 5/5 (>21) | 5/5 (>21) | 5/5 (>21) | ||
| AAHA | 1 | 0/5 (7.6 ± 0.5) | 0/5 (9.2 ± 1.6) | 5/5 (>21) | 5/5 (>21) | 10× less |
| 2 | 0/5 (7.6 ± 1.1) | 0/5 (9.0 ± 1.2) | 4/5 (8.0) | 5/5 (>21) | ||
| AAAH | 1 | 6/6 (>21) | 6/6 (>21) | 5/5 (>21) | 5/5 (>21) | 100× less |
| Quadruple mutation | ||||||
| AAAA | 1 | 0/5 (8.0 ± 1.0) | 5/5 (>21) | 5/5 (>21) | 5/5 (>21) | 100× less |
| 2 | 5/6 (11.0) | 6/6 (>21) | 6/6 (>21) | 6/6 (>21) | ||
a
Number of surviving mice/number of mice in group. MDD, mean day of death of mice that died within 21 days of infection.
b
Compared to WT virus.