TABLE 3.
Body weight changes and lung infection parameters during infection with WT and mutant virusesf
| Virusa | Mean % body wt change (g)b | Lung virus titer (day 2 p.i.) (% of WT PFU) | Lung parameters (day 6)
|
|||
|---|---|---|---|---|---|---|
| Consolidation scorec | Histopathologyd
|
|||||
| Lung wt (g) | Lesions | Hemorrhage | ||||
| WT | ||||||
| CCHH | −15.8 | (5.3 ± 0.9) × 108 (100)e | 2.4 ± 0.2 | 226 ± 28 | +/++ | +/++ |
| Double mutants | ||||||
| ACAH | +0.8 | (9.6 ± 0.7) × 106* (1.8) | 0.5 ± 0.4** | 143 ± 30** | − | +/++ |
| AAHH | −2.5 | (4.1 ± 0.3) × 107* (7.7) | 0.9 ± 0.3** | 185 ± 31 | +/+++ | −/+ |
| Triple mutants | ||||||
| CAAA | −19.0 | (4.9 ± 0.5) × 107* (9.2) | 2.1 ± 0.3 | 195 ± 6 | +/++ | −/+ |
| ACAA | −15.6 | (1.1 ± 0.8) × 107* (2.1) | 1.8 ± 0.8 | 210 ± 52 | + | −/+ |
| AAHA | −3.6 | (7.3 ± 0.7) × 107* (13.8) | 1.9 ± 0.8 | 203 ± 26 | ++ | −/+ |
| AAAH | +11.3 | (1.1 ± 0.1) × 102** (<0.001) | 0.0 ± 0.0** | 120 ± 21** | − | + |
| Quadruple mutant | ||||||
| AAAA | +11.9 | (6.8 ± 0.1) × 101** (<0.001) | 0.0 ± 0.0** | 123 ± 13** | − | + |
a
The infecting virus dose was 105 PFU/mouse.
b
Between day 0 and day 6 of infection (groups of five mice).
c
Consolidation score is based upon the overall appearance of lungs on day 6. A higher score (range, 0 to 4) indicates more consolidation.
d
Severity scores: −, no lesions or hemorrhage; +, mild; ++, moderate; +++, severe. The results represent the average from groups of five mice.
e
Percentage of WT (CCHH virus) PFU.
f
*P < 0.05; **P < 0.01 compared to WT (CCHH virus).