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. 2006 Jun;80(12):5807–5821. doi: 10.1128/JVI.00254-06

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Copyright © 2006, American Society for Microbiology

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FIG. 5. — MP does not colocalize with actin. (A) MP^wt-transfected cells were stained with antibodies specific for MP and with BODIPY-558/568-conjugated phalloidin for actin. MP shows microtubule-specific distribution (top left) and no spatial overlap with actin (top right). Treatment of cells with cytochalasin D affects the pattern and function of actin (bottom right) without changing the circular-filamentous pattern of MP distribution (bottom left), indicating that this distribution is actin independent. (B) MP^wt-transfected cells treated with latrunculin B and stained with antibodies for MP (left) and α-tubulin (right). Microfilament disruption is evident in untransfected cells, which show clear effects on cell shape (right, top and bottom cells). In MP^wt-transfected cells, both cell shape and the association of MP^wt with microtubules are not affected. Bar = 10 μm.