TABLE 3.
Polymerase(s) | Replication capacitya | Transient replicon IC50 (μM)
|
HCV NS5B570-BK IC50 (μM)c
|
NNI-1 KD (μM)d | |
---|---|---|---|---|---|
NNI-1b | NNI-2b | NNI-1 | |||
WT | 1 | 0.3 ± 0.03 | 0.4 ± 0.06 | 0.14 ± 0.04 | 0.02 |
L419M | 0.8 ± 0.13 | 15 ± 2.4 | 0.4 ± 0.05 | 11.0 ± 4.4 | 0.23 |
M423T | 1.0 ± 0.21 | 8.4 ± 0.9 | 0.3 ± 0.04 | 15.0 ± 2.1 | 0.44 |
M423I | 0.25 ± 0.02 | 4.4 ± 0.8 | 0.3 ± 0.07 | NDe | ND |
I482L | 0.45 ± 0.16 | 16.6 ± 3.5 | ND | ND | ND |
L419M/M423T | 0.19 ± 0.05 | 25 ± 2.4 | ND | ND | ND |
Replication capacity compared to the WT in the absence of inhibitor.
IC50 values represent the averages ± standard errors of means of the results from at least three independent experiments.
IC50 values represent the averages ± standard errors of means of the results from at least three independent experiments using the cIRES template.
KD values were determined using Biacore technology as detailed in Materials and Methods.
ND, not determined.