TABLE 8.
Phenotypic characterization of dual-resistance replicons
| Polymerase(s) | Replication capacitya | Transient replicon assay IC50b
|
|
|---|---|---|---|
| NNI-1(μM) | NNI-3 (nM) | ||
| WT | 1 | 0.3 ± 0.03 | 2.6 ± 0.3 |
| M423T | 1 ± 0.21 | 8.4 ± 0.9 | 1.6 ± 0.2 |
| I482L | 0.45 ± 0.16 | 16.6 ± 3.5 | 1.7 ± 0.4 |
| M414L | 1.24 ± 0.23 | 0.3 ± 0.03 | 50 ± 9 |
| M414L/M423T | 0.7 ± 0.12 | 7.5 ± 0.9 | 44 ± 7.5 |
| M414L/M423T/I482L | 0.7 ± 0.13 | >33 | 40 ± 10 |
a
Replication capacity compared to the WT in the absence of inhibitor.
b
The IC50 values for NNI-1 and NNI-3 represent the averages ± standard errors of the means of the results from three independent experiments.