FIG. 4.

Uncleaved hammerhead sequences are eliminated from the 5′ ends of the BDV and MV cRNA during viral replication. Viruses rescued from plasmids pBRPol II-H/HmutrBDVc and pBRPol II-H/HmutrMV, respectively, were propagated in Vero cells, and BDV and MV particles were isolated and purified as described previously (1, 23). Sequences were determined by ligation of a synthetic RNA oligonucleotide (Oligo) to the 5′ termini of isolated RNA molecules and subsequent reverse transcription-PCR amplification. Shown are sequences from near the 5′ terminus of the cRNA (black line) including part of the synthetic RNA oligonucleotide (gray line). Numbering indicates the nucleotide position in the electropherogram. Note that the electropherogram shows the complementary strand.