FIG. 6.

Blocking expression of the 11-kDa protein dose not influence relative abundance of major viral transcripts. (A) Schematic diagram of the B19 genome and probe used. The promoter (p6), the intron donors (D1 and D2), and acceptors (A1-1, A1-2, and A2) are shown. The locations of the PNSVP probe and the expected protected bands are depicted. (B) RPA analysis of B19 transcripts. Twenty micrograms of total RNA isolated at 72 h posttransfection of UT7/Epo-S1 cells was hybridized with probe PNSVP, and the sizes of the protected fragments are indicated by arrows on the right. The molecular marker is a ³²P-labeled RNA ladder (Ambion) with the respective sizes indicated in nucleotides on the left.