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Localization of K1271 to the mitochondria. HEK293 cells were transfected with combinations of 200 ng of IKKɛ and RIG-I; in some cases, cells were also infected with Sendai virus (40 hemagglutinating units/ml) at 24 h posttransfection for a total of 12 h. Mitochondrial extracts were prepared, and 50 μg of extract was run on 7.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotted with anti-K1271 antibody.
