Fig. 6.

Virulence of C. albicans pyk1/pyk1, pck1/pck1 and icl1/icl1 null mutants in the mouse model of systemic infection.

A. Survival of immunocompetent female BALB/c mice following tail vein injection of C. albicans cells: open squares, first control representing the parental strain, RM1000 containing CIp20 (CLM19-3: Table 1); closed circles, experiment representing the homozygous null mutant containing CIp20 (icl1/icl1, CLM25-5; pck1/pck1, CLM56-4; pyk1/pyk1, CLM44-5: Table 1); open circles, second control representing the null mutant containing CIp20 and the wild-type gene (ICL1, CLM26-1; PCK1, CLM57-2; PYK1, CLM45-9: Table 1).

B. Tissue burdens in mouse organs infected with the same C. albicans strains. Fungal colony forming units per gram of tissue were measured in the left kidney (black), right kidney (grey) and brain (white).