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. 2006 Jun 2;2(6):e84. doi: 10.1371/journal.pgen.0020084

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A–F) Six serial sections of a metaphase II centromere. Conventional technique. The outer plates of both sister kinetochores (arrowheads) are clearly differentiated.

(G and H) Two sections of metaphase II centromeres after the Os-PPD technique. The condensed chromosomes show a very low contrast, while sister kinetochores show a high contrast. The kinetochores appear joined by a continuous band of medium contrast material (arrows).

(I) Centromeric region of a chromosome in late metaphase II. A low contrast material extends as a thin and discontinuous band (arrows) joining the highly contrasted sister kinetochores.

Bars, 0.5 μm (A–F), 2.5 μm (G,H), and 2.5 μm (I).