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Novel proteins identified in the proteomic screens cosediment with ribosomes in an EDTA-dependent manner. Anti-TAP Western blots on sucrose gradient fractions in the absence (left) or presence (right) of EDTA. The locations of the ribosomal subunits in the gradients are indicated in the chromatograms and by Western blots of RPL3 and ASC1. CDC28 marks the nonribosomal fractions, and NOP1 shows the migration of a preribosomal complex
