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. 2006 May 15;20(10):1294–1307. doi: 10.1101/gad.1422006

Figure 5.

Figure 5.

Human MCT-1 interacts with DRP1 and complements Δtma20 phenotypes. (A) α-DRP1 (top) or α-Flag (bottom) Western blots of cell lysates (Lys) and immunoprecipitates (IPs) from HEK293 cells transfected with Flag vector or Flag-MCT1. The lysate is 1/20 of the whole-cell extract used for the IP. (B) The amount of translation through nonsense mutations in a β-galactosidase reporter gene in wild-type (WT) and Δtma20 strains transfected with either empty expression vector (p413-GPD), yeast TMA20 (pTMA20), or the human MCT-1 gene expressed under control of the yeast GPD promoter (pGPD-MCT1). Error is reported as the standard deviation. (C) Polysome profiles from a wild-type yeast strain transfected with the empty pRS416 expression vector (left), the Δtma20 strain transfected with the empty pRS416 expression vector (middle), or Δtma20 transfected with an expression vector containing yeast TMA20 (pTMA20) (right). (D) Wild-type strain transfected with p416-GPD (left), Δtma20 strain transfected with p416-GPD (middle), or the Δtma20 strain transfected with pGPD-MCT1 (right). The arrow indicates the varying size of the 40S peak in the different profiles.