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. 2001 Jan 23;98(3):1188–1193. doi: 10.1073/pnas.021558898

Figure 1.

Figure 1

Dicoumarol affects p53 stability. (A) HCT116 cells were incubated without (−) or with 400 μM dicoumarol for 90 and 180 min. (B) HCT116 cells were γ-irradiated at 6 Gy and incubated without (−) or with 200 and 400 μM dicoumarol for 4 h. This blot was underexposed compared with A to highlight the increase in p53 protein level after irradiation. (C) COS 1 cells were γ-irradiated at 6 Gy and incubated without (−) or with 200 and 400 μM dicoumarol for 4 h. Protein extraction and immunoblot analysis were carried out as described in Materials and Methods, with the use of Pab 1801 monoclonal anti-p53 antibody. The blots were then stripped and reprobed with monoclonal anti-β-tubulin antibody as a control for equal protein loading in each lane. Dic., dicoumarol.