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. 2001 Jan 23;98(3):1188–1193. doi: 10.1073/pnas.021558898

Figure 6.

Figure 6

Degradation of mutant and wild-type p53 by dicoumarol but not by other antiapoptotic agents such as IL-6 or thapsigargin. M1-t-p53 cells were cultured for 6 h without (−) or with 10 nM thapsigargin, 50 ng/ml IL-6, or 100 μM dicoumarol. (A) At 32°C, when p53 behaves like wild type. (B) At 37°C, when p53 behaves like a mutant. Immunoblot analysis was carried out with Pab 240 monoclonal anti-p53 antibody. The blots were then stripped and re-probed with anti-IκB and anti-β-tubulin antibody. Dic., dicoumarol.