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. Author manuscript; available in PMC: 2007 Mar 1.
Published in final edited form as: J Am Soc Nephrol. 2006 Feb 1;17(3):637–647. doi: 10.1681/ASN.2005050534

Figure 5.

Figure 5

PI3-K inhibition prevents PC-1-induced tubulogenesis. (a through q) PC-1-induced tubulogenesis is inhibited by PI3-K inhibition. MDCK and MDCKPKD1Zeo cell lines were grown suspended in a collagen gel as described previously (22) either in the absence (-) or the presence (+) of 15 μM LY294002 or 25 nM Wortmannin for 2 d (a through d and I through m), 4 d (e through h), or 8 d (n through q). Representative examples of the control cell lines (a, b, e, f, i, l, n, and o) and MDCKPKD1Zeo cell lines (c, d, g, h, k, m, p, and q) are shown. Photographs were taken at ×40 magnification; a through m were magnified further ×4. (r) Quantification of the experiments described in a was performed as described previously (22). The structures observed in three to five wells for each clone were counted. Treatment in the presence of 15 μM LY294002 or 25 nM Wortmannin dramatically reduced, although not completely suppressed, the total number of tubules observed in MDCKPKD1Zeo cultures.