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. Author manuscript; available in PMC: 2006 Jun 2.
Published in final edited form as: Dev Dyn. 2005 Dec;234(4):878–891. doi: 10.1002/dvdy.20565

Figure 6. Direct versus indirect targets of SOX7 regulation.

Figure 6

Fertilized eggs were injected with RNA encoding GR-SOX7-GFP (0.5 ng/embryo). Animal caps were treated in four different ways: either left in standard media (AC) with 1% ethanol (the carrier for the dexamethasone), incubated with 20 μM dexamethasone (+DEX), pretreated with 100 μg/ml emetine for 30 minutes and then incubated with 20mM dexamethasone and 100 μg/ml emetine (+DEX +Eme), or incubated with 100 μg/ml emetine alone (+Eme). In contrast to cycloheximide (see Sinner et al 2004), emetine treatment had no apparent effect on the expression of any of the genes examined in the absence of dexamethasone. In the presence of dexamethasone, the Xnrs were induced, along with Eomes, Hex, Wnt11 and Cerberus; of these genes, Xnr4, Xnr5 and Xnr6 were expressed in the presence of both dexamethasone and emetine, indicating that they are direct targets of SOX7 regulation.