PR and AR expression in coronary arteries, aorta, and primary coronary VMC from untreated intact male RMs. A, Double-labeling ICC showing the expression of AR and PR in 5-μm cross-sections of coronary artery from intact male RM. B, Double-labeling ICC showing the expression of AR and PR in 5-μm cross-sections of aorta from intact male RM. C, Single-labeling ICC showing the expression of AR and PR in coronary VMC. The data in A and B are representative results from three cross-sections from untreated intact male RM, and the data in C are representative of results from untreated (control) male RM coronary VMC from three coverslips. D, Western blotting showed the expression of AR and PR in coronary VMC from male RM. PR was detected as a 84-kDa band using the rat monoclonal antibody (JZB39 Greene), whereas AR was detected as a 120-kDa band using the rabbit polyclonal antibody (PG-21). The data shown represent two independent experiments.