Fig. 3.

Effects of MPA and DHT on TP receptor expression in aorta. ICC showing expression of TP receptors in 5-μm cross-sections of aorta from untreated (control), MPA-treated, and DHT-treated intact male RM. TP receptor was labeled with a custom primary polyclonal chicken antibody, and secondary labeling was performed with a secondary, rhodamine-tagged, antichicken IgY. Imaging of immunolabeled coronary VMC was performed with a C-Apo ×40/1.2 water immersion on a Zeiss Axiovert 200M microscope with EB-CCD camera (Hamamatsu Photonics). The data shown are representative results from three cross-sections sampled in two monkeys in each of the three groups.