Fig. 4.

Effects of P, MPA, and DHT on TP receptor expression in primary coronary VMC. ICC by indirect immunofluorescence showing expression of TP receptors in untreated and treated coronary VMC: A, untreated control; B, P treatment (1 nm); C, DHT treatment (10 nm); D, MPA treatment (10 nm). Imaging of immunolabeled coronary VMC was performed with a C-Apo ×40/1.2 water immersion on a Zeiss Axiovert 200M microscope with an EB-CCD camera (Hamamatsu Photonics). The data shown are representative of results from three independent experiments.