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Northern blot analysis of gcHIF-1α and gcHIF-4α . A representative Northern blot derived from the tissues of one normoxic and one hypoxic fish from a total of five in each group is shown. Total RNA (20 μg) samples from various tissues of grass carp exposed to normoxia (N) and hypoxia (H) for 4 and 96 h were analysed by Northern hybridization using SL18 (gcHIF1α), SL12 (gcHIF4α) and a 115-bp grass carp 28S rDNA fragment as probes.
