Figure 1.

ERM expression in adult rat sciatic and optic nerves. (A) Confocal images of adult rat sciatic nerves double stained for ankyrin (green) and EBP50 (teased fibers; red) or ezrin (frozen sections; red). EBP50 and ezrin are concentrated in a collar of staining around the axolemma at the node of Ranvier, which is stained specifically with ankyrin antibodies; overlap of staining appears yellow in the merged images. The rotated images (right, Middle and Bottom) show that ezrin and EBP50 form a ring just external to that of ankyrin. (Bar = 10 μm.) (B) Teased sciatic nerve from P11 stained with ezrin (red) and Caspr (green) antibodies. In addition to its localization at the node of Ranvier, ezrin can also be detected in the outer mesaxonal spiral (arrowheads); Caspr is concentrated in the paranodes. (Bar = 10 μm.) (C) Confocal image of adult rat optic nerve double stained for ezrin (red) and ankyrin (green). In the CNS, ezrin is found in elongated astrocytic processes not associated with central nodes. (Bar = 10 μm.)