Desensitization due to prolonged application of 1 mM ACh on receptor.
(A) A typical recording from an oocyte expressing
αL251C and wild-type β, γ, and δ, with
IPEAK, IPLAT, and
IPOST indicated. (B) The
fraction of current remaining at the end of the desensitizing
application of 1 mM ACh,
IPLAT/IPEAK,
for each mutant and wild type. (C) The degree of
recovery of current after a 45-s washout of 1 mM ACh,
IPOST/IPEAK,
for each mutant and wild type. Means ± SEM and numbers of
experiments are shown.