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. Author manuscript; available in PMC: 2006 Jun 5.
Published in final edited form as: J Immunother. 2005;28(4):403–411. doi: 10.1097/01.cji.0000170363.22585.5a

FIGURE 3.

FIGURE 3

IL-2 incubation results in increased CD25 and FOXP3 expression in pre-separation CD4+ T cells but not CD25-depleted CD4+ T cells. A, CD25 is not re-expressed on CD25-depleted CD4 cells after 3 days in IL-2 culture. CD4-enriched pre-separation and CD25-depleted cells, and non-enriched CD25+ fraction cells were cultured in the presence of 100 CU/mL rhIL-2 for 72 hours and assessed for CD25 using PE-conjugated CD25 4A3 clone antibody or isotype control. Cells were gated through a viable CD4+ gating. B. Foxp3 expression remained low in CD25-depleted CD4+ T cells after IL-2 culture. FOXP3 expression was measured by TaqMan RT-PCR before and after 72 hours of IL-2 incubation. Results represent the relative number of FOX3 copies per 104 copies of β-actin ± SEM.