TABLE 2.
Lymphocyte-Gated Cells |
||||||
---|---|---|---|---|---|---|
Donor | %CD3+of Total | % CD3+ | % CD8+of CD3 | % CD4+of CD3 | % CD4+CD25+ | |
1 | Pre-separation | 51 | 78 | 22 | 70 | 52 |
Post-CD25+ fraction | 84 | 91 | 7 | 91 | 56 | |
Post-CD25− fraction | 45 | 75 | 36 | 55 | <1 | |
2 | Pre-separation | 55 | 77 | 65 | 33 | 61 |
Post-CD25+ fraction | 83 | 88 | 16 | 82 | 67 | |
Post-CD25− fraction | 51 | 72 | 79 | 17 | <1 |
Pre- and post-CD25 depletion donor 1 and 2 pheresis samples were blocked with normal mouse immunoglobulin before staining with mouse anti-CD3, CD4, CD8, or CD25 antibodies and viable cells selected through PI gating. For CD25 staining, primary biotin-labeled antibody staining was followed by secondary anti-biotin antibody staining. Values correspond to the percentage of positive events within the total cell population or lymphocyte-gated population as indicated.