TABLE 2.

Phenotypic Analysis of Pheresis Products After CD25 Depletion

			Lymphocyte-Gated Cells
Donor		%CD3+of Total	% CD3+	% CD8+of CD3	% CD4+of CD3	% CD4+CD25+
1	Pre-separation	51	78	22	70	52
	Post-CD25+ fraction	84	91	7	91	56
	Post-CD25− fraction	45	75	36	55	<1
2	Pre-separation	55	77	65	33	61
	Post-CD25+ fraction	83	88	16	82	67
	Post-CD25− fraction	51	72	79	17	<1

Pre- and post-CD25 depletion donor 1 and 2 pheresis samples were blocked with normal mouse immunoglobulin before staining with mouse anti-CD3, CD4, CD8, or CD25 antibodies and viable cells selected through PI gating. For CD25 staining, primary biotin-labeled antibody staining was followed by secondary anti-biotin antibody staining. Values correspond to the percentage of positive events within the total cell population or lymphocyte-gated population as indicated.