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. 2006 Jun 5;103(24):9327–9332. doi: 10.1073/pnas.0603601103

Fig. 4.

Fig. 4.

Circadian rhythms of PER2::LUCIFERASE in SCN explants in response to medium change. (A) Representative bioluminescence rhythm in an SCN explant from PER2::LUC-wild-type mice. In this record, a medium change was administered at CT8 that resulted in no phase shift. The arrow indicates when the medium change occurred. (B) Representative bioluminescence rhythm in an SCN explant from PER2::LUC-Clock/+ mice. In this record, medium change was administered at CT10 that resulted in ≈2-h phase delay. (C and D) Phase–response curve to medium changes in SCN explants from PER2::LUC-wild-type (n = 43) (C) and PER2::LUC-Clock/+ (n = 51) (D) mice. The x axis indicates the CT when the medium changes occurred as determined from the preceding free-running period of PER2::LUC bioluminescence, with the peak of the rhythm defined as CT12. The y axis indicates the phase shift (in hours) produced by the medium change. (E and F) Phase–transition curve to the medium changes in SCN explants from PER2::LUC-wild-type (E) and PER2::LUC-Clock/+ (F) mice. Data from C and D are replotted so that the x axis indicates the old phase (CT) when the medium change occurred as determined from the preceding free-running period of the bioluminescence. The y axis indicates the new phase (CT) of the medium change calculated from the reset rhythm.