Fig. 4.

Matrix modulators are perturbed in S3KO. (A) Immunoprecipitations with tissue extracts performed with specific integrin complex antibodies, αVβ3 and αVβ5, and immunoblotted with αV antibody. (B) Wound margins analyzed for TGFβ1 at 24 h by immunostaining (a) and quantification (b). Red bar represents means; n = 5; ∗, P < 0.05. (Scale bar: 125 μm.) (C) Time course substrate-specific zymography for MMP2 and MMP9 (gelatin; Upper) or MMP12 (casein; Lower). (D) In situ zymography on cryosections using fluorescent substrate overlays 12 h (a) and 21 days (b) after wounding. (Scale bars: 200 μm.) Boxed areas show prominent enzymatic activity.