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MALDI-TOF analysis of N-glycans from E15 embryos. (A) Wild type, (B) MX-null, (C) MII-null, and (D) MII/MX double-null. N-glycans were released from embryo homogenates by peptide N-glycosidase F digestion and were permethylated before MALDI-TOF analysis. Annotations are based on compositional information provided by MALDI molecular weights, complemented by collisional activation tandem MS and linkage analysis experiments (data not shown).
