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. 2006 Jun 5;103(24):8983–8988. doi: 10.1073/pnas.0603248103

Fig. 5.

Fig. 5.

Enzymatic activity of recombinant MX in vivo and in vitro. (A) Rescue of complex-type N-glycan synthesis by α-mannosidases in MII/MX double-null fibroblasts. MII/MX double-null fibroblasts were transfected with vector alone (mock), vector encoding mouse MII cDNA (mMII), or vector encoding mouse MX cDNA (mMX). Transfected cells were probed with E-PHA lectin to detect complex-type N-glycans. (B) Enzymatic hydrolysis of N-glycan oligosaccharides with recombinant α-mannosidases. Each PA-tagged oligosaccharide, M5.1, M6.1, and H5.11 was incubated with a soluble form of the respective enzyme synthesized and secreted from COS-1 cells, and the digest was analyzed by amide-column HPLC. The elution position of the starting material and final product (100.2) is shown by the solid and dashed arrows, respectively.