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. Author manuscript; available in PMC: 2006 Jun 5.
Published in final edited form as: Neuron Glia Biol. 2004 Nov;1(4):307–316. doi: 10.1017/S1740925X05000190

Fig. 2.

Fig. 2

Neuronal loading of NP-EGTA in NP-EGTA-AM-loaded slices is insignificant. (A1) A pyramidal neuron was loaded with potassium salts of NP-EGTA (2 mM, pre-loaded with 2 mM Ca2+) and fluo-4 (0.1 mM) through conventional whole-cell recording. Uncaging NP-EGTA induced Ca2+ elevation as shown by the change in fluo-4 fluorescence. Scale bar, 15 μm. (A2) Time-course of ΔF/F0 of the experiment shown in A1. (A3) Uncaging NP-EGTA induced Ca2+-activated K+ current in the pyramidal neuron. (B) Perforated whole-cell recordings from a pyramidal neuron in NP-EGTA-AM-loaded slices. UV flashing to the neuron did not activate Ca2+-activated K+ currents.