Table 5. Distribution of CH, TCA, and TCOH in Liver.

Male and female F344 rats (175–225 g body weight) were exposed to one of three doses of TRI (2, 5, 15 mmol/kg body weight in 1 ml corn oil) by oral gavage. Animals were kept in metabolic cages for up to 48 hr. Due to the nature of the tissue or fluid sampling, each time point was represented by a separate animal for each time dose and point. Results are pmol/g tissue and are means ± SE of measurements from 3 animals for each data point. CH, TCA, and TCOH concentrations in liver homogenates were measured by GC with electron capture detection. The limits of detection were 0.05, 1.5, and 0.05 pmol/g tissue, respectively.

	2 mmol TRI/kg		5 mmol TRI/kg		15 mmol TRI/kg
	Male	Female	Male	Female	Male	Female
CH
2 hr	0.90 ± 0.41a,d,g	0.80 ± 0.13d	0.27 ± 0.03e	0.08 ± 0.02b,h	0.55 ± 0.01a,i	0.29 ± 0.06e
4 hr	0.49 ± 0.06a,i	0.10 ± 0.03b,h	1.01 ± 0.35d,g	< 0.05c	3.80 ± 1.36j	0.27 ± 0.07e
8 hr	0.12 ± 0.03b	< 0.05c	1.55 ± 0.01f	0.09 ± 0.02b,h	0.13 ± 0.04b	0.23 ± 0.06e
24 hr	< 0.05c	0.49 ± 0.05a,i	0.72 ± 0.14d	0.07 ± 0.01h	0.75 ± 0.51d,i	0.20 ± 0.03e
48 hr	< 0.05c	< 0.05c	1.17 ± 0.15g	0.11 ± 0.01b	0.19 ± 0.10b.e	< 0.05c
TCA
2 hr	< 1.5a	< 1.5a	< 1.5a	4.84 ± 1.51e,f	1.50 ± 0.46a	2.70 ± 0.87d
4 hr	< 1.5a	< 1.5a	< 1.5a	< 1.5a	< 1.5a	< 1.5a
8 hr	1.75 ± 0.39a,d	< 1.5a	< 1.5a	< 1.5a	4.99 ± 0.70f	< 1.5a
24 hr	3.36 ± 0.50b	< 1.5a	2.57 ± 1.67a,b,d,e	< 1.5a	< 1.5a	< 1.5a
48 hr	9.11 ± 1.22c	2.70 ± 0.92b,d,e	< 1.5a	< 1.5a	< 1.5a	< 1.5a
TCOH
2 hr	< 0.05a	< 0.05a	< 0.05a	< 0.05a	0.09 ± 0.06a	< 0.05a
4 hr	< 0.05a	< 0.05a	0.31 ± 0.10b	< 0.05a	< 0.05a	< 0.05a
8 hr	< 0.05a	< 0.05a	0.57 ± 0.05c	< 0.05a	< 0.05a	< 0.05a
24 hr	< 0.05a	< 0.05a	0.23 ± 0.05b	< 0.05a	< 0.05a	< 0.05a
48 hr	< 0.05a	< 0.05a	0.41 ± 0.06d	< 0.05a	< 0.05a	< 0.05a

Values for a given metabolite with different superscript letters are significantly different (P < 0.05) from one another.