. Author manuscript; available in PMC: 2006 Jul 1.

Published in final edited form as: J Toxicol Environ Health A. 2006 Jul;69(13):1285–1309. doi: 10.1080/15287390500360133

Table 9. Concentrations of glutathione and glutathione disulfide in liver.

Male and female F344 rats (175–225 g body weight) were exposed to one of three doses of TRI (2, 5, 15 mmol/kg body weight in 1 ml corn oil) by oral gavage. Animals were kept in metabolic cages for up to 48 hr. Due to the nature of the tissue or fluid sampling, each time point was represented by a separate animal. Results are nmol/g tissue and are means ± SE of measurements from 3 animals for each data point. GSH and GSSG concentrations in liver homogenates were measured by HPLC after derivatization with iodoacetate and 1-fluoro-2,4-dinitrobenzene. The limit of detection was 11 nmol/g tissue.

	2 mmol TRI/kg		5 mmol TRI/kg		15 mmol TRI/kg
	Male	Female	Male	Female	Male	Female
GSH
2 hr	3160 ± 254^a	1290 ± 80^d,l	3153 ± 161^a	1740 ± 140^i,o	2354 ± 270^b,g	1580 ± 280^d,e,f,i
4 hr	2345 ± 92^b,g	1380 ± 180^d,e	2650 ± 283^a,b,n	1240 ± 150^d,j,k,l	1687 ± 126^i,o	2250 ± 110^b,m,n
8 hr	1961 ± 143^c,i,m	1630 ± 130^e,f,i,p	1997 ± 300^c,f,g,i,m	1680 ± 190^i,o	2256 ± 144^b	1530 ± 90^e,o
24 hr	2275 ± 93^b,c	1530 ± 160^e,i,k	807 ± 68^h	1010 ± 180^h,l	1656 ± 195^e,i,p	1800 ± 100^o,p
48 hr	3005 ± 354^a	1510 ± 240^e,i,k,p	954 ± 192^h,j	1710 ± 140^i,o	1579 ± 225^d,e,f,i,o	2420 ± 250^b
GSSG
2 hr	64.7 ± 5.8^a	40.0 ± 11.6^c,e	82.7 ± 2.2^b,f	43.3 ± 8.8^c	95.0 ± 10.0^f,g	49.3 ± 20.7^a,c
4 hr	79.3 ± 6.2^b,f	46.7 ± 3.3^c	63.3 ± 5.6^a	66.7 ± 3.3^a	64.7 ± 6.7^a	83.3 ± 18.6^b,f
8 hr	88.3 ± 13.3^b,f	80.0 ± 10.0^b,f	22.7 ± 10.7^d	60.0 ± 15.3^a	88.3 ± 6.5^b,f	83.3 ± 8.8^b,f
24 hr	39.0 ± 14.0^c,e	63.3 ± 18.6^a,b	26.3 ± 8.1^d,e	43.3 ± 13.3^c,d	58.7 ± 16.0^a,c	56.7 ± 6.7^a,c
48 hr	41.3 ± 8.8^c,e	60.0 ± 10.0^a	18.0 ± 2.1^d	56.7 ± 6.7^a	60.0 ± 10.4^a	120 ± 20^g

Values for either GSH or GSSG with different superscript letters are significantly different (P < 0.05) from one another.