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. 2006 May 31;34(10):2953–2965. doi: 10.1093/nar/gkl349

Figure 3.

Figure 3

Secondary and tertiary structures of wild type and mutant CVB3 3′NCRs. Secondary (A) and tertiary (B) structure of the wt CVB3 3′NCR. The 3′NCR consists of three hairpin domains, X, Y and Z. The structure can be closed by an interaction between the poly(A) with a 4 nt U-stretch overlapping the oriR and the 3D-coding region. The X domain can be stacked to the tertiary ‘kissing’ (K) interaction to form a coaxial helical element, which is connected by single-stranded nucleotide stretches to a second coaxial helical domain Z-Y. (C) Schematic representation of the kissing distortion mutants, KD1 and KD2, in which 6 and 4 bp, respectively, were mutated (underlined). (D) Partial 3′NCR sequence of a virus recovered after transfection with the KD1 mutant. The inserted cytosine residue in the recovered mutant is underlined. (E) The entire 3′NCR sequence of a virus recovered after transfection with the KD2 mutant.