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Time to CPE after transfection or infection with CVB3 oriR mutants
| Construct/mutation | Days before CPE | |
|---|---|---|
| Post transfection | Post passage | |
| p53CB3/T7 (wildtype) | 1 | nd |
| p53CB3/T7-ΔX | — | 4 |
| p53CB3/T7-ΔY | — | 4 |
| pRibCB3/T7 (wildtype) | <1 | nd |
| pRibCB3/T7-ΔXΔY | 4/5 | nd |
| pRibCB3/T7-Δ3′NCR | 5 | nd |
A total of 5 × 106 cells were transfected with ∼4µg of RNA. ‘—’ means not detectable CPE; nd means not done.
