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. 2006 May 31;34(10):2906–2913. doi: 10.1093/nar/gkl368

Figure 4.

Figure 4

Hybridase activity of hRNase-1. Assays were performed at 37°C in 40 mM Tris–HCl (pH 7.4), containing 60 mM NaCl, 60 mM KCl, 20 µg/ml BSA and 10 nM substrate. Left panel shows representative reaction progress curves obtained with 1.8 nM (b), 2.5 nM (c), 3.2 nM (d) and 5.7 nM (e) hRNase-1. The lowest trace (a) was obtained from a control reaction without enzyme. In this set of assays, the maximum change in fluorescence intensity, obtained by incubation with a large excess of hRNase-1, was 380 fluorescence units. Right panel shows the initial rates of substrate cleavage as a function of concentration of hRNase-1.