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. 2006 May 31;34(10):2906–2913. doi: 10.1093/nar/gkl368

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© The Author 2006. Published by Oxford University Press. All rights reserved

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Michaelis–Menten plot for cleavage of the double-helical substrate by hRNase-1. Assays were performed as detailed in Figure 4, except that substrate concentration was 20–400 nM and enzyme concentration was 0.14–1.4 nM.