Table 1.
Increased genomic instability in polλDN expressing cells
| Clone | Number of metaphases | Cells with aberrations (%) | Aberration type dicentric/fusion (%) breaks (%) | ||
|---|---|---|---|---|---|
| UT | CT2B | 103 | 1.0 | 1.0 | 0.0 |
| R2 | 104 | 2.9 | 1.9 | 1.0 | |
| RD10 | 90 | 7.8 | 6.7 | 1.1 | |
| IR | CT2B | 107 | 8.4 | 6.5 | 1.9 |
| R2 | 122 | 19.7 | 16.4 | 3.3 | |
| RD10 | 94 | 59.5 | 57.1 | 24.6 | |
Metaphase spreads were analysed from untreated (UT) and 2 Gy treated cells (IR). CT2B, R2 and RD10 are independent cell lines transfected with the empty vector, the polλ WT and the polλDN forms, respectively.