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. 2006 May 31;34(10):2914–2924. doi: 10.1093/nar/gkl293

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© The Author 2006. Published by Oxford University Press. All rights reserved

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Life span of various strains containing spilt-chromosomes. (A) Average life spans for FY833 (WT), ID-1, ID-2 and ID-8 strains were 34.1, 31.9, 20.6 and 22.7 generations, respectively. (B) Average life spans for the fob1 mutants, ID-10, ID-11, ID-12 and ID-13 were 42.5, 41.2, 30.1 and 19.9 generations, respectively. (C) Detection of the ERCs. DNA isolated from various strains was subjected to electrophoresis (0.6% agarose gel for 30 h at 1 V/cm) followed by Southern hybridization using 5S rDNA as a probe. Thin arrows represent ERCs and thick arrow indicates hybridization signal of the genomic rDNA copies. M; Lambda DNA/HindIII marker.