Table 2.
Experimental conditions and results
| Enzyme | C U/ml | DNA template | Nsites | NP | N/NP × 100 | <F> | <L> | LS | LL | ρS % |
|---|---|---|---|---|---|---|---|---|---|---|
| BpmI | 20 | ½-λ-L | 17 | 136 | 2.3 (0.42) | 29.6 (11.2) | 777 (208) | 60 | 3634 | 18 (12) |
| BsgI | 30 | ½-λ-L | 31 | 465 | 1.7 (0.22) | 26.9 (12.3) | 645 (83) | 45 | 3344 | −3.2 (12) |
| BspMI | 20 | ½-λ-L | 24 | 276 | 0.91 (0.14) | 23.2 (10.3) | 613 (124) | 25 | 4494 | −16 (9.0) |
| Cfr10I | 100 | ½-λ-L | 56 | 1540 | 1.2 (0.052) | 22.5 (12.4) | 470 (89) | 22 | 7444 | −23 (5.9) |
| Eco57I | 50 | ½-λ-L | 25 | 300 | 2.7 (0.19) | 21.8 (11.2) | 573 (103) | 19 | 7512 | −6.7 (11) |
| EcoRII | 50 | ½-λ-L | 36 | 630 | 0.29 (0.026) | 25.6 (12.2) | 665 (176) | 21 | 6940 | −24 (10) |
| FokI | 40 | LBAC-B | 26 | 325 | 0.79 (0.038) | 14.8 (11.0) | 301 (61) | 18 | 4120 | −22 (5.6) |
| HpaII | 100 | LBAC-B | 49 | 1176 | 0.51 (0.041) | 16.6 (9.92) | 325 (65) | 20 | 2500 | −23 (6.9) |
| Sau3AI | 40 | LBAC-A | 55 | 1485 | 0.66 (0.041) | 25.0 (10.8) | 423 (85) | 7 | 2682 | −11 (5.1) |
Nsites is the number of recognition sites on the DNA template; C is the enzyme concentration in U/ml; NP is the number of pairs of sites that can form loops on the DNA template; N/NP is the number of observed loops per molecule normalized by NP (and standard error); <F> is the mean loop disruption force (and SD) in pN; <L> is the mean observed loop size in base pairs (and SD) for the normalized distributions (see text); LS is the shortest observed event size in the ensemble of data; LL is the longest observed event size; ρS is the correlation coefficient between disruption force and event size for events <150 bp (and the SD expected for uncorrelated data).