Table 1.
Primer(5′→3′) |
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Exon | Forward | Reverse |
Product Size (bp) |
1 | TACAGGGAGTTGAGGTGCCG | ATTCCTGGAAGGGAACCAGAAG | 111 |
2 | TGAAGAGCCCGACAGCTACA | TGAAGAGCCCGACAGCTACA | 468 |
3 | TTAGCTTGTTGAGCAGAGTGGG | TTAAGACGCCTGGTGAGAGGA | 396 |
4 | TCAGTGTTGCTGGGGATGTT | TGAAGCCTGGCTTGAATGG | 426 |
5 | TGGTGAAGCCTTGAGTCCCA | ATGGAGAACTGTGTGGCAGG | 552 |
6 | TTCTTGACCAGCCCAGGTAGA | ATGGAGAACTGTGTGGCAGGA | 284 |
7 | TTCTTCCAGTTCTGGGCAGAGA | TCATCAATGTCTGTGCCAGGG | 438 |
8 | TCTGCCAAGGTACAGGTTGC | ATTCCCATCATCCCTCAGGG | 333 |
9 | ATCTATTCAGACCAGCGAGTGCC | TGTGCATGTCAGTTGAGGGTTG | 401 |
10 | TAGCACACAGGCTGACACAA | TCAGCCACCAGGACTTTCTT | 320 |
Note.— Annealing temperature was 53°C for all amplifications.