Figure 4. .

Genomewide view of brain DNA–HpaII (A) and sperm DNA–HHA (B) data sets. The unmethylated fraction of genomic DNA was enriched from brain DNA (n=22) or sperm samples (n=25), and each was hybridized individually (Cy5), in contrast to the pooled samples (Cy3). The CV of ratio Cy5/Cy3 was calculated for each spot across the 22 or 25 individuals and was mapped to the corresponding genomic location. Each chromosome ideogram is overlaid with red bars that represent the position of each clone on the array. The bars highlighted in green are the loci that showed statistically significant variance (90th percentile).