Figure 7. .

MS-SNuPE analysis of densities of methylated cytosines in CpG dinucleotides of selected genes. Genomic DNA from 11 individuals was treated with sodium bisulphite and then was PCR amplified for each gene. The genes NELL2, SCAM1, NEIL2, MKL2, CDH13, and OLR1 are represented. The methylation status of CpG dinucleotides within each of the restriction-enzyme sites was interrogated using the primer-extension reactions. Methylation of each of the CpG dinucleotides is represented as a percentage of methylated PCR products: completely unmethylated (white circles), partially methylated (partially black circles), or completely methylated (black circles).