Figure 2. .

Location and conservation of mutated residues. A, Location of mutations along schematic representation of pre-pro-desmoglein-2. SS = signal peptide sequence; Pro = propeptide; TM = transmembrane domain; IA = intracellular anchor; IPL = intracellular proline-rich linker; RUD = repeated-unit domains; DTD = desmoglein-specific terminal domain (adapted from the work of Getsios et al.¹⁸). B, ClustalW sequence alignment of human DSG2 to orthologues and to other human desmogleins (DSG1, DSG3, and DSG4) and desmocollins (DSC2, DSC3,and DSC4). Identical residues are shaded in black; conserved residues are shaded in gray. Mutations identified in the present study are shown at the top of each alignment. The furin-recognition motif and plakoglobin binding domain are indicated.