Table 1.

Primer sequences and annealing temperatures used for the PCR

Gene	Sequence 5′–3′	Temp
Mfn1	sense TGT TTT GGT CGC AAA CTC TG	60
	Anti CTG TCT GCG TAC GTC TTC CA
Mfn2	sense ATG CAT CCC CAC TTA AGC AC	60
	Anti CCA GAG GGC AGA ACT TTG TC
PGC-1α	sense TCA GTC CTC ACT GGT GGA CA	62
	Anti TGC TTC GTC GTC AAA AAC AG
ERRα	Sense TTCTCATCGCTGTCGCTGTCT	64
	Anti CAGCCGCCGCACTAGTTG
NRF-1	Sense GGT GCA GCA CCT TTG GAG AA	60
	Anti CCA GAG CAG ACT CCA GGT CTT C
NRF-2	Sense CAA GAA CGC CTT GGG ATA CC	60
	Anti AAA CCA CCC AAT GCA GGA CTT
COXIV	Sense CAT GTG GCA GAA GCA CTA TGT GT	60
	Anti GCC ACC CAC TCT TTG TCA AAG
18S	Sense GAG GAT GAG GTG GAA CGT GT	60
	Anti GGA CCT GGC TGT ATT TTC CA
36B4	Sense GTG ATG TGC AGC TGA TCA AGA CT	60
	Anti GAT GAC CAG CCC AAA GGA GA

Mfn1, mitofusin-1; Mfn2, mitofusin-2; PGC-1α, peroxisome proliferator-activated receptor γ coactivator 1α; ERRα, oestrogen related receptor-α; NRF-1, nuclear respiratory factor-1; NRF-2, nuclear respiratory factor-2; COXIV, cytochrome c oxidase subunit IV; 18S, ribosomal 18S; 36B4, acidic ribosomal phosphoprotein PO.