Figure 6. Level of p53 phosphorylation and caspase-3 activation in the presence of p38 inhibitor in hypertonic medium.

A, RacV₁₂A₃ cells were preincubated for 1 h in isotonic (337 mosmol l⁻¹) medium and 10 μm SB203580 followed by 4.5 h incubation in hypertonic medium (687 mosmol l⁻¹). Cell lysates were prepared and subjected to SDS-PAGE and Western blot analysis. Specific antibodies against phospho-p53 (rabbit antiphospho-p53 S15) and cleaved caspase-3 (rabbit anticleaved caspase-3) were used. B, intensity of phospho-p53 S15 in isotonic medium containing SB203580 and hypertonic medium with and without SB203580 relative to isotonic control (n = 3). C, intensity of cleaved caspase-3 in isotonic medium containing SB203580 and hypertonic medium with and without SB203580 relative to the isotonic control (n = 3). *P < 0.05, **P < 0.01, significantly different from the isotonic control.